Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
11684-2-AP targets PSMB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSMB6 fusion protein Ag2296 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 6 |
| Calculated molecular weight | 239 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC000835 |
| Gene Symbol | PSMB6 |
| Gene ID (NCBI) | 5694 |
| RRID | AB_2172199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28072 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| IHC protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| IF protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats. | ||
J Biol Chem Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes | ||
Front Genet Immune regulation and prognostic prediction model establishment and validation of PSMB6 in lung adenocarcinoma | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) | ||
JHEP Rep Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice |