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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB7 Polyclonal antibody

PSMB7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30283-1-AP

Synonyms

Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB7 Polyclonal antibody (30283-1-AP)

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMB7 Polyclonal antibody (30283-1-AP)

    WB analysis using 30283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Polyclonal antibody (30283-1-AP)

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Polyclonal antibody (30283-1-AP)

    IHC staining of human colon cancer using 30283-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30283-1-AP targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB7 fusion protein Ag32201 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 7
Calculated molecular weight29.9 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC000509
Gene symbol PSMB7
Gene ID (NCBI) 5695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

Protocols

Product Specific Protocols
WB protocol for PSMB7 antibody 30283-1-APDownload protocol
IHC protocol for PSMB7 antibody 30283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols