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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB7 Monoclonal antibody, PBS Only

PSMB7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D7B8

Cat no : 68641-1-PBS

Synonyms

Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB7 Monoclonal antibody (68641-1-Ig)

    WB analysis using 68641-1-Ig (same clone as 68641-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68641-1-Ig (PSMB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68641-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Monoclonal antibody (68641-1-Ig)

    IHC staining of human colon cancer using 68641-1-Ig (same clone as 68641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68641-1-Ig (PSMB7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68641-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Monoclonal antibody (68641-1-Ig)

    IHC staining of human colon cancer using 68641-1-Ig (same clone as 68641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68641-1-Ig (PSMB7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68641-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68641-1-PBS targets PSMB7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB7 fusion protein Ag6544 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 7
Calculated molecular weight29.9 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC000509
Gene symbol PSMB7
Gene ID (NCBI) 5695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)