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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB8 Monoclonal antibody, PBS Only

PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5B6

Cat no : 66759-1-PBS

Synonyms

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB8 Monoclonal antibody (66759-1-Ig)

    WB analysis using 66759-1-Ig (same clone as 66759-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PSMB8 Monoclonal antibody (66759-1-Ig)

    WB analysis of HSC-T6 using 66759-1-Ig (same clone as 66759-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of human oesophagus cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of rat stomach using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of mouse colon using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of mouse stomach using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of rat colon using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of human liver cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IHC staining of human liver cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IF Staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using PSMB8 Monoclonal antibody (66759-1-Ig)

    IF Staining of human liver cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB8 Monoclonal antibody (66759-1-Ig)

    IF Staining of HeLa using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSMB8 Monoclonal antibody (66759-1-Ig)

    IF Staining of HepG2 using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66759-1-PBS targets PSMB8 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB8 fusion protein Ag6780 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated molecular weight 30 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001114
Gene symbol PSMB8
Gene ID (NCBI) 5696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).