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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB9 Monoclonal antibody, PBS Only

PSMB9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C7C8

Cat no : 67748-1-PBS

Synonyms

beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis using 67748-1-Ig (same clone as 67748-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67748-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis of rat spleen using 67748-1-Ig (same clone as 67748-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig (same clone as 67748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig (same clone as 67748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67748-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human spleen using 67748-1-Ig (same clone as 67748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67748-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67748-1-PBS targets PSMB9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB9 fusion protein Ag6802 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Calculated molecular weight 23 kDa
Observed molecular weight20-23 kDa
GenBank accession numberBC065513
Gene symbol PSMB9
Gene ID (NCBI) 5698
RRIDAB_2918517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.