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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMC5 Polyclonal antibody

PSMC5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14752-1-AP

Synonyms

p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMC5 Polyclonal antibody (14752-1-AP)

    WB analysis using 14752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PSMC5 Polyclonal antibody (14752-1-AP)

    WB analysis of NIH/3T3 using 14752-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PSMC5 Polyclonal antibody (14752-1-AP)

    WB analysis of A549 using 14752-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PSMC5 Polyclonal antibody (14752-1-AP)

    WB analysis of HEK-293 using 14752-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMC5 Polyclonal antibody (14752-1-AP)

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMC5 Polyclonal antibody (14752-1-AP)

    IHC staining of human liver cancer using 14752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14752-1-AP targets PSMC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMC5 fusion protein Ag6497 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 5
Calculated molecular weight 46 kDa
Observed molecular weight 45-49 kDa
GenBank accession numberBC002367
Gene symbol PSMC5
Gene ID (NCBI) 5705
RRIDAB_2878077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMC5 antibody 14752-1-APDownload protocol
IHC protocol for PSMC5 antibody 14752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols