PSMC6 Polyclonal antibody

Name: PSMC6 Polyclonal antibody
Brand: Proteintech
SKU: 15839-1-AP

PSMC6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15839-1-AP

Synonyms

CADP44, p42, P44, Proteasome subunit p42, PSMC6, Rpt4, SUG2

Validation Data Gallery

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AllWBIHC

WB analysis using 15839-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15839-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15839-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15839-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15839-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 15839-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15839-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15839-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15839-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 15839-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, human testis tissue, rat liver tissue, mouse liver tissue, rat brain tissue
Positive IHC detected in	human liver cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

15839-1-AP targets PSMC6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PSMC6 fusion protein Ag8589 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, ATPase, 6
Calculated molecular weight	389 aa, 44 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC005390
Gene symbol	PSMC6
Gene ID (NCBI)	5706
RRID	AB_2237797
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.

Protocols

Product Specific Protocols
WB protocol for PSMC6 antibody 15839-1-AP	Download protocol
IHC protocol for PSMC6 antibody 15839-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Biomed Sci Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology. Authors - Yi-Chao Lee View Article
mouse	IHC	FASEB J Glucose-induced decrease of cystathionine β-synthase mediates renal injuries. Authors - Yanting Yu View Article
rat	WB	Cardiovasc Drugs Ther Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway. Authors - Kuixiang Chen View Article
mouse,rat	WB	Acta Pharmacol Sin Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition Authors - Qian Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMC6 Polyclonal antibody

PSMC6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 15839-1-AP

WB analysis of mouse brain using 15839-1-AP

WB analysis of rat liver using 15839-1-AP

WB analysis of human brain using 15839-1-AP

WB analysis of human testis using 15839-1-AP

IHC staining of human liver cancer using 15839-1-AP

IHC staining of human liver cancer using 15839-1-AP

IHC staining of mouse brain using 15839-1-AP

IHC staining of mouse brain using 15839-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.

Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.

Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.

Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition