Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, SKOV-3 cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83521-2-RR targets PSMD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PSMD1 fusion protein Ag12009 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 |
| Calculated molecular weight | 953 aa, 106 kDa |
| Observed molecular weight | 106 kDa |
| GenBank accession number | BC094720 |
| Gene Symbol | PSMD1 |
| Gene ID (NCBI) | 5707 |
| RRID | AB_3671147 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99460 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMD1 antibody 83521-2-RR | Download protocol |
| IHC protocol for PSMD1 antibody 83521-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |