Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 14786-1-AP
WB result of PSMD11 antibody (14786-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD11 transfected HeLa cells.
WB analysis of HeLa using 14786-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14786-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14786-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14786-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 14786-1-AP
IP result of anti-PSMD11 (IP:14786-1-AP, 3ug; Detection:14786-1-AP 1:500) with MCF-7 cells lysate 2000ug.
IHC staining of human stomach cancer using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lung using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 14786-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14786-1-AP
Immunofluorescent analysis of MCF-7 cells using 14786-1-AP (PSMD11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PSMD11 Polyclonal antibody

PSMD11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14786-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

S9, Rpn6, p44.5, 26S proteasome non-ATPase regulatory subunit 11

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, mouse testis tissue, rat brain tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human stomach cancer tissue, human lung tissue, rat lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14786-1-AP targets PSMD11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PSMD11 fusion protein Ag6435 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC000437
Gene Symbol	PSMD11
Gene ID (NCBI)	5717
RRID	AB_2268979
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00231
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.

Protocols

Product Specific Protocols
WB protocol for PSMD11 antibody 14786-1-AP	Download protocol
IHC protocol for PSMD11 antibody 14786-1-AP	Download protocol
IF protocol for PSMD11 antibody 14786-1-AP	Download protocol
IP protocol for PSMD11 antibody 14786-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Authors - Sparks A A View Article
human	WB	Mol Cell Proteomics Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells. Authors - Qi Tong-Gang TG View Article
rat	WB	J Med Chem Target Fishing Reveals a Novel Mechanism of 1,2,4-Oxadiazole Derivatives Targeting Rpn6, a Subunit of 26S Proteasome. Authors - Zhen Dai View Article
mouse	WB	FASEB J Susceptibility of microtubule-associated protein 1 light chain 3β (MAP1LC3B/ LC3B) knockout mice to lung injury and fibrosis. Authors - Vidya Sagar Kesireddy View Article
human	WB	J Cell Biochem Homoharringtonine could induce quick protein synthesis of PSMD11 through activating MEK1/ERK1/2 signaling pathway in pancreatic cancer cells. Authors - Lele Wang View Article
mouse	WB,IF	BMC Dev Biol Generation and identification of a conditional knockout allele for the PSMD11 gene in mice. Authors - Linlin Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14786-1-AP

WB analysis of HeLa using 14786-1-AP

WB analysis of MCF-7 using 14786-1-AP

WB analysis of mouse testis using 14786-1-AP

WB analysis of rat brain using 14786-1-AP

IP experiment of MCF-7 using 14786-1-AP

IHC staining of human stomach cancer using 14786-1-AP

IHC staining of human stomach cancer using 14786-1-AP

IHC staining of rat lung using 14786-1-AP

IHC staining of rat lung using 14786-1-AP

IHC staining of human lung using 14786-1-AP

IHC staining of human lung using 14786-1-AP

IF Staining of MCF-7 using 14786-1-AP

PSMD11 Polyclonal antibody

PSMD11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.

Target Fishing Reveals a Novel Mechanism of 1,2,4-Oxadiazole Derivatives Targeting Rpn6, a Subunit of 26S Proteasome.

Susceptibility of microtubule-associated protein 1 light chain 3β (MAP1LC3B/ LC3B) knockout mice to lung injury and fibrosis.

Homoharringtonine could induce quick protein synthesis of PSMD11 through activating MEK1/ERK1/2 signaling pathway in pancreatic cancer cells.

Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.