Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11412-1-AP targets PSMD12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSMD12 fusion protein Ag1957 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 |
| Calculated molecular weight | 456 aa, 53 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC019062 |
| Gene Symbol | PSMD12 |
| Gene ID (NCBI) | 5718 |
| RRID | AB_10640803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00232 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proteasome 26S subunit, non-ATPase 12 (PSMD12; also known as RPN5) was first discovered in fission yeast. It is a non-ATPase subunit of the 19S regulator of the 26S proteasome complex, which is involved in regulating the cell cycle, repairing DNA damage, and regulating apoptosis by mediating the internal protein balance through the removal of misfolded proteins or damaged intracellular substructures. In addition, PSMD12 contributes to tumor cell growth and apoptosis in breast cancer. (PMID: 33987398)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMD12 antibody 11412-1-AP | Download protocol |
| IHC protocol for PSMD12 antibody 11412-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ann Transl Med PSMD12 promotes glioma progression by upregulating the expression of Nrf2.
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Biochem Biophys Res Commun PSMD12 promotes breast cancer growth via inhibiting the expression of pro-apoptotic genes.
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