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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD12 Polyclonal antibody (11412-1-AP)

    WB analysis using 11412-1-AP (same clone as 11412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11412-1-AP (PSMD12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PSMD12 Polyclonal antibody (11412-1-AP)

    IHC staining of human gliomas using 11412-1-AP (same clone as 11412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11412-1-AP (PSMD12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PSMD12 Polyclonal antibody (11412-1-AP)

    IHC staining of human gliomas using 11412-1-AP (same clone as 11412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11412-1-AP (PSMD12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11412-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PSMD12 Polyclonal antibody, PBS Only

PSMD12 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11412-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11412-1-PBS targets PSMD12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD12 fusion protein Ag1957 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Calculated molecular weight 456 aa, 53 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC019062
Gene Symbol PSMD12
Gene ID (NCBI) 5718
RRIDAB_10640803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00232
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Proteasome 26S subunit, non-ATPase 12 (PSMD12; also known as RPN5) was first discovered in fission yeast. It is a non-ATPase subunit of the 19S regulator of the 26S proteasome complex, which is involved in regulating the cell cycle, repairing DNA damage, and regulating apoptosis by mediating the internal protein balance through the removal of misfolded proteins or damaged intracellular substructures. In addition, PSMD12 contributes to tumor cell growth and apoptosis in breast cancer. (PMID: 33987398)