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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMD14/POH1 Recombinant antibody

PSMD14/POH1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242160E7

Cat no : 84767-3-RR

Synonyms

PSMD14, POH1, PAD1, EC:3.4.19.-, 26S proteasome-associated PAD1 homolog 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD14/POH1 Recombinant antibody (84767-3-RR)

    WB analysis using 84767-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PSMD14/POH1 Recombinant antibody (84767-3-RR)

    IHC staining of mouse heart using 84767-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PSMD14/POH1 Recombinant antibody (84767-3-RR)

    IHC staining of mouse heart using 84767-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PSMD14/POH1 Recombinant antibody (84767-3-RR)

    IF Staining of A375 using 84767-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (84767-3-RR, Clone: 242160E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84767-3-RR

    Affinity and Kinetic Characterization of 84767-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84767-3-RR against Human PSMD14/POH1 were performed. The affinity constant is 3.60 nM.

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Tested Applications

Positive WB detected inA431 cells, K-562 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84767-3-RR targets PSMD14/POH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMD14/POH1 fusion protein Ag2694 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
Calculated molecular weight 35 kDa
Observed molecular weight33 kDa
GenBank accession numberBC009524
Gene symbol PSMD14
Gene ID (NCBI) 10213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

Protocols

Product Specific Protocols
WB protocol for PSMD14/POH1 antibody 84767-3-RRDownload protocol
IHC protocol for PSMD14/POH1 antibody 84767-3-RRDownload protocol
IF protocol for PSMD14/POH1 antibody 84767-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols