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KD/KO Validated

PSMD2 Polyclonal antibody

Name: PSMD2 Polyclonal antibody
Brand: Proteintech
SKU: 14748-1-AP

PSMD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14748-1-AP

Synonyms

26S proteasome subunit p97, P97, Protein 55.11, PSMD2, Rpn1, S2, TRAP2

Validation Data Gallery

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AllWBIPIHC

WB analysis using 14748-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14748-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14748-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14748-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14748-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 14748-1-AP
IP result of anti-PSMD2 (IP:14748-1-AP, 4ug; Detection:14748-1-AP 1:1000) with K-562 cells lysate 3320ug.
IHC staining of human breast cancer using 14748-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14748-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14748-1-AP (PSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14748-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SKOV-3 cells, HeLa cells, K-562 cells, human heart tissue, mouse skeletal muscle tissue, PC-3 cells, A431 cells, HL-60 cells, mouse heart tissue, rat heart tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 11 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 3 publications below

Product Information

14748-1-AP targets PSMD2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PSMD2 fusion protein Ag6484 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Calculated molecular weight	100 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC002368
Gene symbol	PSMD2
Gene ID (NCBI)	5708
RRID	AB_2170472
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Tumor necrosis factor type 1 receptor-associated protein 2 (TRAP2), encoded by PSMD2 gene, is a non-ATPase regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. TRAP2 may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor.

Protocols

Product Specific Protocols
WB protocol for PSMD2 antibody 14748-1-AP	Download protocol
IHC protocol for PSMD2 antibody 14748-1-AP	Download protocol
IP protocol for PSMD2 antibody 14748-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Cancer vulnerabilities unveiled by genomic loss. Authors - Nijhawan Deepak D View Article
human	WB	Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Authors - Sparks A A View Article
human	WB	Cells The Identification of Nuclear FMRP Isoform Iso6 Partners Authors - Nassim Ledoux View Article
human	WB,CoIP	Cell Commun Signal NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma. Authors - Hong-Zhong Zhou View Article
human	WB	BMC Mol Biol PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism. Authors - Yanjie Tan View Article
human,mouse	WB,CoIP	J Exp Clin Cancer Res AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma. Authors - Chunyan Gu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMD2 Polyclonal antibody

PSMD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 14748-1-AP

WB analysis of HeLa using 14748-1-AP

WB analysis of K-562 using 14748-1-AP

WB analysis of human heart using 14748-1-AP

WB analysis of mouse skeletal muscle using 14748-1-AP

IP experiment of K-562 using 14748-1-AP

IHC staining of human breast cancer using 14748-1-AP

IHC staining of human breast cancer using 14748-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cancer vulnerabilities unveiled by genomic loss.

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

The Identification of Nuclear FMRP Isoform Iso6 Partners

NQO1 potentiates apoptosis evasion and upregulates XIAP via inhibiting proteasome-mediated degradation SIRT6 in hepatocellular carcinoma.

PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism.

AHSA1 is a promising therapeutic target for cellular proliferation and proteasome inhibitor resistance in multiple myeloma.