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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMD3 Recombinant antibody

PSMD3 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242470D4

Cat no : 84928-2-RR

Synonyms

TSTA2, S3, RPN3, P58, 26S proteasome non-ATPase regulatory subunit 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD3 Recombinant antibody (84928-2-RR)

    WB analysis using 84928-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using PSMD3 Recombinant antibody (84928-2-RR)

    FC experiment of A549 using 84928-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using PSMD3 Recombinant antibody (84928-2-RR)

    FC experiment of HeLa using 84928-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, U2OS cells, U-251 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84928-2-RR targets PSMD3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMD3 fusion protein Ag2684 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Calculated molecular weight 534 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC012302
Gene symbol PSMD3
Gene ID (NCBI) 5709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

Protocols

Product Specific Protocols
WB protocol for PSMD3 antibody 84928-2-RRDownload protocol
FC protocol for PSMD3 antibody 84928-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols