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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD4 Monoclonal antibody (66179-1-Ig)

    WB analysis using 66179-1-Ig (same clone as 66179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66179-1-Ig (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Monoclonal antibody (66179-1-Ig)

    IHC staining of human testis using 66179-1-Ig (same clone as 66179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Monoclonal antibody (66179-1-Ig)

    IHC staining of human testis using 66179-1-Ig (same clone as 66179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD4 Monoclonal antibody (66179-1-Ig)

    IF Staining of HeLa using 66179-1-Ig (same clone as 66179-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (66179-1-Ig, Clone: 3G1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

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PSMD4 Monoclonal antibody, PBS Only

PSMD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66179-1-PBS
Clone No.3G1A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66179-1-PBS targets PSMD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag7010 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002365
Gene Symbol PSMD4
Gene ID (NCBI) 5710
RRIDAB_2881574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55036
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.