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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMD5 Polyclonal antibody

PSMD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11469-1-AP

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PSMD5 Polyclonal antibody (11469-1-AP)

    WB analysis of HeLa using 11469-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11469-1-AP (PSMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PSMD5 Polyclonal antibody (11469-1-AP)

    WB analysis of Jurkat using 11469-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11469-1-AP (PSMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PSMD5 Polyclonal antibody (11469-1-AP)

    WB analysis of human liver using 11469-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11469-1-AP (PSMD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PSMD5 Polyclonal antibody (11469-1-AP)

    WB analysis of mouse liver using 11469-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11469-1-AP (PSMD5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using PSMD5 Polyclonal antibody (11469-1-AP)

    IP experiment of mouse liver using 11469-1-AP

    IP result of anti-PSMD5 (IP:11469-1-AP, 4ug; Detection:11469-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSMD5 Polyclonal antibody (11469-1-AP)

    IHC staining of human prostate cancer using 11469-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11469-1-AP (PSMD5 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11469-1-AP targets PSMD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD5 fusion protein Ag2026 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014478
Gene symbol PSMD5
Gene ID (NCBI) 5711
RRIDAB_2170648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD5, also named as KIAA0072, belongs to the proteasome subunit S5B/HSM3 family. It acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly, it is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a SMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. (PMID:15489334). This antibody is specific to PSMD5.

Protocols

Product Specific Protocols
WB protocol for PSMD5 antibody 11469-1-APDownload protocol
IHC protocol for PSMD5 antibody 11469-1-APDownload protocol
IP protocol for PSMD5 antibody 11469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols