PSMD5 Monoclonal antibody

PSMD5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F7

Cat no : 67682-1-Ig

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B


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Tested Applications

Positive WB detected inpig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67682-1-Ig targets PSMD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

Tested Reactivity Human, pig, rat, rabbit, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen PSMD5 fusion protein Ag19298 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight50 kDa
GenBank accession numberBC014478
Gene symbol PSMD5
Gene ID (NCBI) 5711
RRIDAB_2882875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMD5 antibody 67682-1-IgDownload protocol
IHC protocol for PSMD5 antibody 67682-1-IgDownload protocol
IF protocol for PSMD5 antibody 67682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols