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- KD/KO Validated
PSMD7 Polyclonal antibody
PSMD7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16034-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
Product Information
16034-1-AP targets PSMD7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSMD7 fusion protein Ag8905 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 |
| Calculated molecular weight | 324 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC012606 |
| Gene symbol | PSMD7 |
| Gene ID (NCBI) | 5713 |
| RRID | AB_2170797 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMD7 antibody 16034-1-AP | Download protocol |
| IHC protocol for PSMD7 antibody 16034-1-AP | Download protocol |
| IF protocol for PSMD7 antibody 16034-1-AP | Download protocol |
| IP protocol for PSMD7 antibody 16034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.
| ||
Cell Signal Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization | ||
Cell Biosci Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradation |
