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  • Featured Product
  • KD/KO Validated

PSMD7 Monoclonal antibody, PBS Only

PSMD7 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E10E7

Cat no : 68188-1-PBS

Synonyms

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD7 Monoclonal antibody (68188-1-Ig)

    WB analysis using 68188-1-Ig (same clone as 68188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PSMD7 Monoclonal antibody (68188-1-Ig)

    WB analysis of HeLa using 68188-1-Ig (same clone as 68188-1-PBS)

    WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68188-1-PBS targets PSMD7 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD7 fusion protein Ag9305 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Calculated molecular weight 324 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012606
Gene symbol PSMD7
Gene ID (NCBI) 5713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)