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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD8 Polyclonal antibody (27504-1-AP)

    WB analysis using 27504-1-AP (same clone as 27504-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27504-1-AP (PSMD8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27504-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMD8 Polyclonal antibody (27504-1-AP)

    IF Staining of U2OS using 27504-1-AP (same clone as 27504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD8 antibody (27504-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27504-1-PBS in a different storage buffer formulation.

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PSMD8 Polyclonal antibody, PBS Only

PSMD8 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27504-1-PBS targets PSMD8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD8 fusion protein Ag26567 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Calculated molecular weight 257 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001164
Gene Symbol PSMD8
Gene ID (NCBI) 5714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48556
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Proteasome 26S Subunit, Non-ATPase (PSMD) gene family, which includes the PSMD1 to PSMD14, regulate ubiqutinated protein breakdown in circulation as well as tumorigenesis. Studies have shown that PSMD8 interacts with the sperm adhesin AQN1 to limit polyfertilization. PSMD8 was more expressed in normal skeletal muscle, cardiac muscle, and tongue muscle; among malignant tumors, PSMD8 was expressed in ovarian cancer, testicular cancer, glioma, and melanoma(PMID:37349676).