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  • Featured Product
  • KD/KO Validated

PSMD9 Polyclonal antibody

PSMD9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 26922-1-AP

Synonyms

Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of A549 using 26922-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of A549 using 26922-1-AP

    WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

  • Western Blot (WB) analysis of mouse spleen tissue using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of mouse spleen using 26922-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of Jurkat using 26922-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of A549 using 26922-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of MCF-7 using 26922-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of MDA-MB-453s using 26922-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    WB analysis of HepG2 using 26922-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using PSMD9 Polyclonal antibody (26922-1-AP)

    IP experiment of A549 using 26922-1-AP

    IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMD9 Polyclonal antibody (26922-1-AP)

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMD9 Polyclonal antibody (26922-1-AP)

    IHC staining of human breast cancer using 26922-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMD9 Polyclonal antibody (26922-1-AP)

    IF Staining of U2OS using 26922-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using PSMD9 Polyclonal antibody (26922-1-AP)

    FC experiment of HeLa using 26922-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, Jurkat cells, mouse spleen tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26922-1-AP targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD9 fusion protein Ag25638 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Calculated molecular weight 27 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC004213
Gene symbol PSMD9
Gene ID (NCBI) 5715
RRIDAB_2880687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

Protocols

Product Specific Protocols
WB protocol for PSMD9 antibody 26922-1-APDownload protocol
IHC protocol for PSMD9 antibody 26922-1-APDownload protocol
IF protocol for PSMD9 antibody 26922-1-APDownload protocol
IP protocol for PSMD9 antibody 26922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols