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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD9 Monoclonal antibody (67338-1-Ig)

    WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PSMD9 Monoclonal antibody (67338-1-Ig)

    WB analysis of A549 using 67338-1-Ig (same clone as 67338-1-PBS)

    WB result of PSMD9 antibody (67338-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSMD9 Monoclonal antibody (67338-1-Ig)

    WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:2800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMD9 Monoclonal antibody (67338-1-Ig)

    IHC staining of human cervical cancer using 67338-1-Ig (same clone as 67338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMD9 Monoclonal antibody (67338-1-Ig)

    IHC staining of human cervical cancer using 67338-1-Ig (same clone as 67338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMD9 Monoclonal antibody (67338-1-Ig)

    IF Staining of U2OS using 67338-1-Ig (same clone as 67338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMD9 Monoclonal antibody (67338-1-Ig)

    IF Staining of HeLa using 67338-1-Ig (same clone as 67338-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PSMD9 Monoclonal antibody (67338-1-Ig)

    FC experiment of HeLa using 67338-1-Ig (same clone as 67338-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD9 Monoclonal antibody (67338-1-Ig, Clone:1H2G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PSMD9 Monoclonal antibody, PBS Only

PSMD9 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67338-1-PBS
Clone No.1H2G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9, 1H2G1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67338-1-PBS targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD9 fusion protein Ag25654 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
Calculated molecular weight 27 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC004213
Gene Symbol PSMD9
Gene ID (NCBI) 5715
RRIDAB_2882596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00233
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.