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  • KD/KO Validated

PSME3 Polyclonal antibody

PSME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14907-1-AP

Synonyms

REG GAMMA, PA28gamma, PA28g, PA28 gamma, Ki nuclear autoantigen


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Tested Applications

Positive WB detected inCOLO 320 cells, mouse spleen tissue, HepG2 cells, Caco-2 cells, HCT 116 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14907-1-AP targets PSME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSME3 fusion protein Ag6702 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC001423
Gene symbol PSME3
Gene ID (NCBI) 10197
RRIDAB_2171098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

Protocols

Product Specific Protocols
WB protocol for PSME3 antibody 14907-1-APDownload protocol
IHC protocol for PSME3 antibody 14907-1-APDownload protocol
IF protocol for PSME3 antibody 14907-1-APDownload protocol
IP protocol for PSME3 antibody 14907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Lett

Proteasome activator subunit 3 promotes pancreatic cancer growth via c-Myc-glycolysis signaling axis.

Authors - Jiefang Guo
humanIHC

EBioMedicine

Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.

Authors - Zhen Yu
  • KD Validated
humanWB

Retrovirology

Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.

Authors - Doueiri Rami R
humanWB,IHC

PeerJ

REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.

Authors - Zhiqiang Yin
  • KD Validated
IF

Nat Aging

Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes

Authors - Hyun Ju Lee
humanWB,IHC

Am J Transl Res

The oncogenic role of REG γ is exerted by activating the Wnt/β-catenin signaling pathway in osteosarcoma

Authors - Zhiqiang Yin
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Laetitia (Verified Customer) (03-21-2024)

Finding antibodies specific to avian proteins is very difficult. I have tried different antibodies from different companies so far. This antibody is by far the best (cost efficient as well)

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Epithelial chicken cells