Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, mouse spleen tissue, HepG2 cells, Caco-2 cells, HCT 116 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
Product Information
14907-1-AP targets PSME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSME3 fusion protein Ag6702 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC001423 |
| Gene Symbol | PSME3 |
| Gene ID (NCBI) | 10197 |
| RRID | AB_2171098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61289 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSME3 antibody 14907-1-AP | Download protocol |
| IHC protocol for PSME3 antibody 14907-1-AP | Download protocol |
| IF protocol for PSME3 antibody 14907-1-AP | Download protocol |
| IP protocol for PSME3 antibody 14907-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Proteasome activator subunit 3 promotes pancreatic cancer growth via c-Myc-glycolysis signaling axis. | ||
EBioMedicine Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.
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Retrovirology Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses. | ||
PeerJ REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.
| ||
Nat Aging Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes | ||
Am J Transl Res The oncogenic role of REG γ is exerted by activating the Wnt/β-catenin signaling pathway in osteosarcoma
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