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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSME3 Polyclonal antibody

PSME3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30009-1-AP

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using PSME3 Polyclonal antibody (30009-1-AP)

    WB analysis of Caco-2 using 30009-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 30009-1-AP (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30009-1-AP targets PSME3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSME3 fusion protein Ag31969 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001423
Gene symbol PSME3
Gene ID (NCBI) 10197
RRIDAB_2923628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

Protocols

Product Specific Protocols
WB protocol for PSME3 antibody 30009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols