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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSME3 Monoclonal antibody, PBS Only

PSME3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8E8

Cat no : 67629-1-PBS

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSME3 Monoclonal antibody (67629-1-Ig)

    WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSME3 Monoclonal antibody (67629-1-Ig)

    WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSME3 Monoclonal antibody (67629-1-Ig)

    IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSME3 Monoclonal antibody (67629-1-Ig)

    IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67629-1-PBS targets PSME3 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSME3 fusion protein Ag6716 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Calculated molecular weight 30 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001423
Gene symbol PSME3
Gene ID (NCBI) 10197
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.