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  • Featured Product
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PSMF1 Polyclonal antibody

PSMF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12941-1-AP

Synonyms

hPI31, PI31, PSMF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMF1 Polyclonal antibody (12941-1-AP)

    WB analysis using 12941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PSMF1 Polyclonal antibody (12941-1-AP)

    WB analysis of HEK-293 using 12941-1-AP

    WB result of PSMF1 antibody (12941-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMF1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of human kidney tissue using PSMF1 Polyclonal antibody (12941-1-AP)

    WB analysis of human kidney using 12941-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PSMF1 Polyclonal antibody (12941-1-AP)

    WB analysis of human heart using 12941-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, human heart tissue, Jurkat cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12941-1-AP targets PSMF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMF1 fusion protein Ag4011 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC029836
Gene symbol PSMF1
Gene ID (NCBI) 9491
RRIDAB_2268855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).

Protocols

Product Specific Protocols
WB protocol for PSMF1 antibody 12941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.

Authors - Hongli An
View Article