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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMG3 Polyclonal antibody

PSMG3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15601-1-AP

Synonyms

PAC-3, PAC3, PAC 3, hPAC3, C7orf48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMG3 Polyclonal antibody (15601-1-AP)

    WB analysis using 15601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Polyclonal antibody (15601-1-AP)

    WB analysis using 15601-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Polyclonal antibody (15601-1-AP)

    WB analysis using 15601-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PSMG3 Polyclonal antibody (15601-1-AP)

    IHC staining of human stomach cancer using 15601-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PSMG3 Polyclonal antibody (15601-1-AP)

    IHC staining of human stomach cancer using 15601-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMG3 Polyclonal antibody (15601-1-AP)

    IHC staining of human liver cancer using 15601-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMG3 Polyclonal antibody (15601-1-AP)

    IHC staining of human liver cancer using 15601-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15601-1-AP targets PSMG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMG3 fusion protein Ag7984 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) assembly chaperone 3
Calculated molecular weight 13 kDa
Observed molecular weight 13-18 kDa
GenBank accession numberBC004308
Gene symbol PSMG3
Gene ID (NCBI) 84262
RRIDAB_2878156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.

Protocols

Product Specific Protocols
WB protocol for PSMG3 antibody 15601-1-APDownload protocol
IHC protocol for PSMG3 antibody 15601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols