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  • Featured Product
  • KD/KO Validated

PTBP1 Polyclonal antibody

PTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55181-1-AP

Synonyms

PTB 1, PTB, pPTB, HNRPI, HNRNPI

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PTBP1 Polyclonal antibody (55181-1-AP)

    WB analysis of HepG2 using 55181-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55181-1-AP (PTBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PTBP1 Polyclonal antibody (55181-1-AP)

    WB analysis of HeLa using 55181-1-AP

    WB result of PTBP1 antibody (55181-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTBP1 Polyclonal antibody (55181-1-AP)

    IHC staining of mouse brain using 55181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55181-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTBP1 Polyclonal antibody (55181-1-AP)

    IHC staining of mouse brain using 55181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55181-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PTBP1 Polyclonal antibody (55181-1-AP)

    IF Staining of mouse brain using 55181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55181-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PTBP1 Polyclonal antibody (55181-1-AP)

    IF Staining of HEK-293 using 55181-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55181-1-AP (PTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55181-1-AP targets PTBP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polypyrimidine tract binding protein 1
Calculated molecular weight 60 kDa
Observed molecular weight 55-62 kDa
GenBank accession numberNM_002819
Gene symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_11182384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Protocols

Product Specific Protocols
WB protocol for PTBP1 antibody 55181-1-APDownload protocol
IHC protocol for PTBP1 antibody 55181-1-APDownload protocol
IF protocol for PTBP1 antibody 55181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
View Article