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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTBP1 Monoclonal antibody (67462-1-Ig)

    WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTBP1 Monoclonal antibody (67462-1-Ig)

    WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

    WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PTBP1 Monoclonal antibody (67462-1-Ig)

    IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PTBP1 Monoclonal antibody, PBS Only

PTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67462-1-PBS
Clone No.3D6D8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67462-1-PBS targets PTBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTBP1 fusion protein Ag28404 相同性解析による交差性が予測される生物種
Full Name polypyrimidine tract binding protein 1
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC013694
Gene Symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_2882692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26599
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.