PTCD3 Polyclonal antibody
PTCD3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 25158-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse embryo tissue, HEK-293 cells, rat heart tissue, rat kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
The immunogen of 25158-1-AP is PTCD3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PTCD3 fusion protein Ag18330 相同性解析による交差性が予測される生物種 |
| Full Name | Pentatricopeptide repeat domain 3 |
| Calculated molecular weight | 689 aa, 79 kDa |
| Observed molecular weight | 78-80 kDa |
| GenBank accession number | BC011832 |
| Gene symbol | PTCD3 |
| Gene ID (NCBI) | 55037 |
| RRID | AB_2879931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| IF protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| IP protocol for PTCD3 antibody 25158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness. | ||
Biomedicines Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema | ||
J Gen Virol Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors |
