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PTCH1 Polyclonal antibody

PTCH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

HPE7, NBCCS, Patched, patched homolog 1 (Drosophila), Patched1, Protein patched homolog 1, PTC, PTC1, PTCH, PTCH1, PTCH11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17520-1-AP targets PTCH1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name patched homolog 1 (Drosophila)
Calculated molecular weight 161 kDa
Observed molecular weight 161 kDa
GenBank accession numberNM_000264
Gene Symbol PTCH1
Gene ID (NCBI) 5727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13635
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.

Protocols

Product Specific Protocols
WB protocol for PTCH1 antibody 17520-1-APDownload protocol
IHC protocol for PTCH1 antibody 17520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
mouseWB

Nat Commun

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.

Authors - Bo Yan
mouseIHC

Am J Hum Genet

Genomic analyses reveal mutational signatures and frequently altered genes in esophageal squamous cell carcinoma.

Authors - Ling Zhang
humanWB

Elife

Activation of Hedgehog signaling in mesenchymal stem cells induces cartilage and bone tumor formation via Wnt/β-Catenin.

Authors - Qi Deng
mouseWB

J Mol Cell Biol

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Authors - Yuwei Li
humanWB

Cancers (Basel)

RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines

Authors - Matea Kurtović
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