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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTDSS1 Polyclonal antibody (20820-1-AP)

    WB analysis using 20820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20820-1-AP (PTDSS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTDSS1 Polyclonal antibody (20820-1-AP)

    IF Staining of HepG2 using 20820-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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PTDSS1 Polyclonal antibody

PTDSS1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20820-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PSS1, PSSA, PSS-1, Phosphatidylserine synthase 1, KIAA0024

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, unboiled HEK-293 cells, HeLa cells, unboiled HeLa cells
Positive IF/ICC detected inHepG2 cells

It is best to use unboiled samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20820-1-AP targets PTDSS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTDSS1 fusion protein Ag14170 相同性解析による交差性が予測される生物種
Full Name phosphatidylserine synthase 1
Calculated molecular weight 473 aa, 56 kDa
Observed molecular weight38~40 kDa
GenBank accession numberBC002376
Gene Symbol PTDSS1
Gene ID (NCBI) 9791
RRIDAB_3669350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTDSS1 gene encodes the enzyme phosphatidylserine synthase 1 (PSS1), which catalyzes the production of phosphatidylserine (PS), a membrane phospholipid involved in different cellular processes such as development, cell signaling, apoptosis, and blood coagulation (PMID: 35224839). PTDSS1 is highly enriched in mitochondria-associated membranes (MAM) and is largely excluded from the bulk of the endoplasmic reticulum (ER). Low apparent molecular mass of 40 kDa compared with the size predicted was attributed to the exceptionally high content of hydrophobic amino acids in PSS1 (PMID: 10938271).

Protocols

Product Specific Protocols
WB protocol for PTDSS1 antibody 20820-1-APDownload protocol
IF protocol for PTDSS1 antibody 20820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols