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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTEN Recombinant antibody, PBS Only

PTEN Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240134F7

Cat no : 80718-7-PBS

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTEN Recombinant antibody (80718-7-RR)

    IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PTEN Recombinant antibody (80718-7-RR)

    FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80718-7-PBS

    Affinity and Kinetic Characterization of 80718-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 80718-7-PBS is PTEN Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTEN fusion protein Ag17274 相同性解析による交差性が予測される生物種
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
GenBank accession numberBC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.