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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTEN Recombinant antibody (80718-7-RR)

    IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PTEN Recombinant antibody (80718-7-RR)

    FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80718-7-PBS

    Affinity and Kinetic Characterization of 80718-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

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PTEN Recombinant antibody, PBS Only

PTEN Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 80718-7-PBS
Clone No.240134F7

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80718-7-PBS targets PTEN in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTEN fusion protein Ag17274 相同性解析による交差性が予測される生物種
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
GenBank accession numberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP60484
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.