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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PTEN Monoclonal antibody

PTEN Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C10B6

Cat no : CL594-60300

Synonyms

EC:3.1.3.-, BZS, BC005821, Bannayan Zonana syndrome, 5C10B6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated PTEN Monoclonal antibody (CL594-60300)

    IF Staining of HepG2 using CL594-60300

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60300 (PTEN antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated PTEN Monoclonal antibody (CL594-60300)

    FC experiment of HepG2 using CL594-60300

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PTEN (CL594-60300, Clone:5C10B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60300 targets PTEN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274 相同性解析による交差性が予測される生物種
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
RRIDAB_2883458
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 PTEN antibody CL594-60300Download protocol
Standard Protocols
Click here to view our Standard Protocols