PTGDS Polyclonal antibody

Name: PTGDS Polyclonal antibody
Brand: Proteintech
SKU: 10754-2-AP

PTGDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10754-2-AP

Synonyms

Beta trace protein, Cerebrin 28, LPGDS, PDS, PGD2, PGD2 synthase, PGDS, PGDS2, Prostaglandin D2 synthase, Prostaglandin H2 D isomerase, PTGDS

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 10754-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 10754-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 10754-2-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10754-2-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10754-2-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10754-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10754-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10754-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 10754-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10754-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10754-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human kidney tissue, human plasma, human heart tissue, human colon tissue, PC-3 cells, human urine tissue
Positive IHC detected in	mouse brain tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 3 publications below

Product Information

10754-2-AP targets PTGDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTGDS fusion protein Ag1163 相同性解析による交差性が予測される生物種
Full Name	prostaglandin D2 synthase 21kDa (brain)
Calculated molecular weight	21 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC005939
Gene symbol	PTGDS
Gene ID (NCBI)	5730
RRID	AB_2174610
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PGDS(Prostaglandin-H2 D-isomerase) is also named as PTGDS, PDS, PGDS2. It catalyzes the conversion of PGH2 to PGD2, and is a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. It has two N-glycosylation recognition sites.

Protocols

Product Specific Protocols
WB protocol for PTGDS antibody 10754-2-AP	Download protocol
IHC protocol for PTGDS antibody 10754-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway. Authors - Hui-Yuan Su View Article
pig	WB	Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. Authors - Yongjie Xu View Article
human	WB	Cell Cycle MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling. Authors - Ailan Yin View Article
human	IHC	Cancer Manag Res Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation. Authors - Ruoyao Zou View Article
human	WB	Med Sci Monit Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma. Authors - Kim Jae Ho JH View Article
mouse	IHC	Mol Neurodegener MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease Authors - Lu Geng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGDS Polyclonal antibody

PTGDS Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 10754-2-AP

WB analysis using 10754-2-AP

WB analysis of human kidney using 10754-2-AP

WB analysis of human heart using 10754-2-AP

WB analysis of human colon using 10754-2-AP

WB analysis of PC-3 using 10754-2-AP

IHC staining of human lung cancer using 10754-2-AP

IHC staining of human lung cancer using 10754-2-AP

IHC staining of mouse brain using 10754-2-AP

IHC staining of mouse brain using 10754-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.

Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.

Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.

MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease