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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGER3 Polyclonal antibody

PTGER3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24761-1-AP

Synonyms

EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PTGER3 Polyclonal antibody (24761-1-AP)

    WB analysis of mouse brain using 24761-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using PTGER3 Polyclonal antibody (24761-1-AP)

    WB analysis of mouse cerebellum using 24761-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using PTGER3 Polyclonal antibody (24761-1-AP)

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PTGER3 Polyclonal antibody (24761-1-AP)

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24761-1-AP is PTGER3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGER3 fusion protein Ag21542 相同性解析による交差性が予測される生物種
Full Name prostaglandin E receptor 3 (subtype EP3)
Calculated molecular weight 43 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC024229
Gene symbol PTGER3
Gene ID (NCBI) 5733
RRIDAB_2879710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTGER3 antibody 24761-1-APDownload protocol
IHC protocol for PTGER3 antibody 24761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Dev Cell

Chromatin remodeling of prostaglandin signaling in smooth muscle enables mouse embryo passage through the female reproductive tract

Authors - Qiliang Xin
View Article