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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGER4 Monoclonal antibody, PBS Only

PTGER4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A2A12

Cat no : 66921-1-PBS

Synonyms

EP4R, EP4, 4A2A12

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    WB analysis of pig brain using 66921-1-Ig (same clone as 66921-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    WB analysis of rat brain using 66921-1-Ig (same clone as 66921-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    WB analysis of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PTGER4 Monoclonal antibody (66921-1-Ig)

    WB analysis of Jurkat using 66921-1-Ig (same clone as 66921-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PTGER4 Monoclonal antibody (66921-1-Ig)

    WB analysis of K-562 using 66921-1-Ig (same clone as 66921-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PTGER4 Monoclonal antibody (66921-1-Ig)

    IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PTGER4 Monoclonal antibody (66921-1-Ig)

    FC experiment of HepG2 using 66921-1-Ig (same clone as 66921-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PTGER4 (66921-1-Ig, Clone:4A2A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66921-1-PBS targets PTGER4 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PTGER4 fusion protein Ag19262 相同性解析による交差性が予測される生物種
Full Name prostaglandin E receptor 4 (subtype EP4)
Calculated molecular weight 488 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC101534
Gene symbol PTGER4
Gene ID (NCBI) 5734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.