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KD/KO Validated

PTGES2 Polyclonal antibody

Name: PTGES2 Polyclonal antibody
Brand: Proteintech
SKU: 10881-1-AP

PTGES2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10881-1-AP

Synonyms

mPGES 2, mPGE synthase-2, Membrane-associated prostaglandin E synthase-2, GBF1, EC:5.3.99.3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 10881-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10881-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10881-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 10881-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10881-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10881-1-AP (PTGES2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 10881-1-AP
IP result of anti-PTGES2 (IP:10881-1-AP, 4ug; Detection:10881-1-AP 1:500) with mouse heart tissue lysate 4000ug.
IHC staining of human breast cancer using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10881-1-AP (PTGES2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10881-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10881-1-AP (PTGES2 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10881-1-AP
Immunofluorescent analysis of Hela cells, using PTGES2 antibody 10881-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 10881-1-AP
Immunofluorescent analysis of HepG2 cells using 10881-1-AP (PTGES2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10881-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HepG2 cells, human stomach tissue, mouse heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 5 publications below

Product Information

10881-1-AP targets PTGES2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, caenorhabditis elegans
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTGES2 fusion protein Ag1329 相同性解析による交差性が予測される生物種
Full Name	prostaglandin E synthase 2
Calculated molecular weight	42 kDa
Observed molecular weight	30-35 kDa, 45 kDa
GenBank accession number	BC011613
Gene symbol	PTGES2
Gene ID (NCBI)	80142
RRID	AB_2175066
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

Protocols

Product Specific Protocols
WB protocol for PTGES2 antibody 10881-1-AP	Download protocol
IHC protocol for PTGES2 antibody 10881-1-AP	Download protocol
IF protocol for PTGES2 antibody 10881-1-AP	Download protocol
IP protocol for PTGES2 antibody 10881-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Brain Behav Immun Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease. Authors - Linbin Dai View Article
rat	WB	Int Immunopharmacol Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. Authors - Shuang Xiao View Article
human	WB,IHC	Tumour Biol Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer. Authors - Jieqi Ke KD Validated View Article
rat	WB	J Neuroimmunol Prostaglandin E2 increases the expression of cyclooxygenase-2 in cultured rat microglia. Authors - Takayuki Nagano View Article
Caenorhabditis elegans	WB	Int J Radiat Biol Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field. Authors - Yongyan Sun View Article
human	IHC	Oncol Lett Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer. Authors - Jieqi Ke View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGES2 Polyclonal antibody

PTGES2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10881-1-AP

WB analysis of HepG2 using 10881-1-AP

WB analysis of mouse heart using 10881-1-AP

WB analysis of human stomach using 10881-1-AP

WB analysis of HEK-293 using 10881-1-AP

IP experiment of mouse heart using 10881-1-AP

IHC staining of human breast cancer using 10881-1-AP

IHC staining of human colon cancer using 10881-1-AP

IHC staining of human colon cancer using 10881-1-AP

IHC staining of human colon cancer using 10881-1-AP

IHC staining of human heart using 10881-1-AP

IHC staining of human heart using 10881-1-AP

IF Staining of HeLa using 10881-1-AP

IF Staining of HepG2 using 10881-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.

Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug.

Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer.

Prostaglandin E2 increases the expression of cyclooxygenase-2 in cultured rat microglia.

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer.