PTGES3 Polyclonal antibody

Name: PTGES3 Polyclonal antibody
Brand: Proteintech
SKU: 15216-1-AP

PTGES3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15216-1-AP

Synonyms

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of rat brain using 15216-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 15216-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15216-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15216-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 15216-1-AP
IP result of anti-PTGES3 (IP:15216-1-AP, 3ug; Detection:15216-1-AP 1:300) with mouse heart tissue lysate 4000ug.
IHC staining of human lung cancer using 15216-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15216-1-AP (PTGES3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15216-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15216-1-AP (PTGES3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15216-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15216-1-AP (PTGES3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15216-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15216-1-AP (PTGES3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 15216-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 15216-1-AP
Immunofluorescent analysis of HeLa cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15216-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat brain tissue, mouse brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

15216-1-AP targets PTGES3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTGES3 fusion protein Ag7384 相同性解析による交差性が予測される生物種
Full Name	prostaglandin E synthase 3 (cytosolic)
Calculated molecular weight	19 kDa
Observed molecular weight	21-23 kDa
GenBank accession number	BC003005
Gene symbol	PTGES3
Gene ID (NCBI)	10728
RRID	AB_2284772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Protocols

Product Specific Protocols
WB protocol for PTGES3 antibody 15216-1-AP	Download protocol
IHC protocol for PTGES3 antibody 15216-1-AP	Download protocol
IF protocol for PTGES3 antibody 15216-1-AP	Download protocol
IP protocol for PTGES3 antibody 15216-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Death Differ Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis. Authors - Yujia Fan View Article
human	WB	Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. Authors - Liyuan Zhang View Article
mouse	WB	Heliyon The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells. Authors - Yoshika Iwata View Article
human	WB	Sci Adv PES1 is a critical component of telomerase assembly and regulates cellular senescence. Authors - Long Cheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTGES3 Polyclonal antibody

PTGES3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of rat brain using 15216-1-AP

WB analysis of rat heart using 15216-1-AP

WB analysis of mouse brain using 15216-1-AP

WB analysis of mouse heart using 15216-1-AP

IP experiment of mouse heart using 15216-1-AP

IHC staining of human lung cancer using 15216-1-AP

IHC staining of human lung cancer using 15216-1-AP

IHC staining of human lung cancer using 15216-1-AP

IHC staining of human lung cancer using 15216-1-AP

IF Staining of A549 using 15216-1-AP

IF Staining of HeLa using 15216-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells.

PES1 is a critical component of telomerase assembly and regulates cellular senescence.