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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTGR1 Polyclonal antibody (13374-1-AP)

    WB analysis using 13374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PTGR1 Polyclonal antibody (13374-1-AP)

    WB analysis of HeLa using 13374-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using PTGR1 Polyclonal antibody (13374-1-AP)

    WB analysis of human colon using 13374-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PTGR1 Polyclonal antibody (13374-1-AP)

    WB analysis of human liver using 13374-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTGR1 Polyclonal antibody (13374-1-AP)

    WB analysis using 13374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PTGR1 Polyclonal antibody (13374-1-AP)

    IP experiment of HepG2 using 13374-1-AP

    IP result of anti-PTGR1 (IP:13374-1-AP, 4ug; Detection:13374-1-AP 1:500) with HepG2 cells lysate 3200ug.

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PTGR1 Polyclonal antibody

PTGR1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13374-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

LTB4DH, PGR1, PRG 1, prostaglandin reductase 1, PTGR1, ZADH3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human colon tissue, human liver tissue, mouse liver tissue, HepG2 cells, rar liver tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13374-1-AP targets PTGR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGR1 fusion protein Ag4196 相同性解析による交差性が予測される生物種
Full Name prostaglandin reductase 1
Calculated molecular weight 329 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC035228
Gene Symbol PTGR1
Gene ID (NCBI) 22949
RRIDAB_2173213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTGR1(Prostaglandin reductase 1) is also named as LTB4DH(NADP-dependent leukotriene B4 12-hydroxydehydrogenase) and belongs to the NADP-dependent oxidoreductase L4BD family. It catalyses the conversion of leukotriene B4 into biologically less reactive 12-oxo-leukotriene B4, a key step for its inactivation and it is a key oxidation step in the process of metabolic inactivation of leukotriene B(4) in various non-leukocyte tissues(PMID:8576264).

Protocols

Product Specific Protocols
WB protocol for PTGR1 antibody 13374-1-APDownload protocol
IP protocol for PTGR1 antibody 13374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Modulation of Nitro-Fatty Acid Signaling: Prostaglandin Reductase-1 is a Nitroalkene Reductase.

Authors - Vitturi Dario A DA
View Article
humanWB

Mol Cancer Ther

Conditional Dependency of LP-184 on Prostaglandin Reductase 1 is Synthetic Lethal in Pancreatic Cancers with DNA Damage Repair Deficiencies

Authors - Diana Restifo
View Article
humanWB

Int J Biol Sci

NUCB2 promotes hepatocellular carcinoma cell growth and metastasis by activating the E2F4/PTGR1 axis

Authors - Yuan Wang
View Article