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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COX-1/Cyclooxygenase-1 Monoclonal antibody

COX-1/Cyclooxygenase-1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G7

Cat no : 67346-1-Ig

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PTGS1 Monoclonal antibody (67346-1-Ig)

    WB analysis of L02 using 67346-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using PTGS1 Monoclonal antibody (67346-1-Ig)

    WB analysis of human peripheral blood leukocyte using 67346-1-Ig

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PTGS1 Monoclonal antibody (67346-1-Ig)

    WB analysis of K-562 using 67346-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PTGS1 Monoclonal antibody (67346-1-Ig)

    WB analysis of A431 using 67346-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTGS1 Monoclonal antibody (67346-1-Ig)

    IF Staining of HeLa using 67346-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inL02 cells, K-562 cells, A431 cells, Human peripheral blood leukocyte
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67346-1-Ig targets COX-1/Cyclooxygenase-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen COX-1/Cyclooxygenase-1 fusion protein Ag28280 相同性解析による交差性が予測される生物種
Full Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 60-72 kDa
GenBank accession numberBC029840
Gene symbol PTGS1
Gene ID (NCBI) 5742
RRIDAB_2882604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Protocols

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
IF protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols