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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYK2 Polyclonal antibody

PYK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10044-2-AP

Synonyms

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PYK2 Polyclonal antibody (10044-2-AP)

    WB analysis of mouse brain using 10044-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PYK2 Polyclonal antibody (10044-2-AP)

    WB analysis of mouse brain using 10044-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PYK2 Polyclonal antibody (10044-2-AP)

    WB analysis of Jurkat using 10044-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PYK2 Polyclonal antibody (10044-2-AP)

    WB analysis of mouse brain using 10044-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10044-2-AP (PYK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using PYK2 Polyclonal antibody (10044-2-AP)

    IHC staining of human medulloblastoma using 10044-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 10044-2-AP (PYK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PYK2 Polyclonal antibody (10044-2-AP)

    IHC staining of human kidney using 10044-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10044-2-AP (PYK2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PYK2 Polyclonal antibody (10044-2-AP)

    IHC staining of human kidney using 10044-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10044-2-AP (PYK2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IHC detected inhuman medulloblastoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10044-2-AP targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYK2 fusion protein Ag0002 相同性解析による交差性が予測される生物種
Full Name PTK2B protein tyrosine kinase 2 beta
Calculated molecular weight 120 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC036651
Gene symbol PTK2B
Gene ID (NCBI) 2185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several introcellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis.

Publications

SpeciesApplicationTitle
ratWB

Herz

Ginsenoside-Rb3 inhibits endothelial-mesenchymal transition of cardiac microvascular endothelial cells.

Authors - L Yang
View Article