Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, rat spleen tissue, mouse spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
18697-1-AP targets PTK6/BRK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | PTK6 protein tyrosine kinase 6 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC035843 |
| Gene Symbol | PTK6 |
| Gene ID (NCBI) | 5753 |
| RRID | AB_2284691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRK, also named as PTK6 and FLJ42088, is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. PTK6 can phosphorylate KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. This antibody is specific to PTK6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTK6/BRK antibody 18697-1-AP | Download protocol |
| IHC protocol for PTK6/BRK antibody 18697-1-AP | Download protocol |
| IF protocol for PTK6/BRK antibody 18697-1-AP | Download protocol |
| IP protocol for PTK6/BRK antibody 18697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Res RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.
| ||
Chin J Cancer The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer. | ||
Dose Response Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer. | ||
Adv Sci (Weinh) ETV4 Mediated Tumor-Associated Neutrophil Infiltration Facilitates Lymphangiogenesis and Lymphatic Metastasis of Bladder Cancer
|