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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTK7 Recombinant antibody (85075-1-RR)

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTK7 Recombinant antibody (85075-1-RR)

    WB analysis using 85075-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85075-1-RR (PTK7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85075-1-RR

    Affinity and Kinetic Characterization of 85075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85075-1-RR against Human PTK7 were performed. The affinity constant is 2.60 nM.

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PTK7 Recombinant antibody

PTK7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85075-1-RR
Clone No.242720A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, CCK4, CCK 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, C2C12 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85075-1-RR targets PTK7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTK7 fusion protein Ag12026 相同性解析による交差性が予測される生物種
Full Name PTK7 protein tyrosine kinase 7
Calculated molecular weight 1070 aa, 118 kDa
Observed molecular weight140 kDa
GenBank accession numberBC071557
Gene Symbol PTK7
Gene ID (NCBI) 5754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13308
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).

Protocols

Product Specific Protocols
WB protocol for PTK7 antibody 85075-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols