PTN Polyclonal antibody

fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27117-1-AP (PTN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

U-87 MG (WT and PTN KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

U-87 MG lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).