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PTP1B Polyclonal antibody

Name: PTP1B Polyclonal antibody
Brand: Proteintech
SKU: 11334-1-AP

PTP1B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11334-1-AP

Synonyms

PTP 1B, PTP1B, PTPN1

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis of Sp2/0 using 11334-1-AP
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11334-1-AP
WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.
WB analysis using 11334-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11334-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 11334-1-AP
THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 11334-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 11334-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11334-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11334-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11334-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11334-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 11334-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11334-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 11334-1-AP
IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.
IHC staining of human prostate cancer using 11334-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens).
IF Staining of BxPC-3 using 11334-1-AP
Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11334-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Sp2/0 cells, BxPC-3 cells, A431 cells, HeLa cells, Jurkat cells, THP-1 cells, mouse liver tissue, human testis tissue, HL-60 cells, human placenta tissue, C6 cells, NIH/3T3 cells, mouse placenta tissue, rat liver tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 32 publications below
IHC	See 7 publications below
IF	See 6 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

11334-1-AP targets PTP1B in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTP1B fusion protein Ag1878 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 1
Calculated molecular weight	435 aa, 50 kDa
Observed molecular weight	45-55 kDa
GenBank accession number	BC015660
Gene symbol	PTPN1
Gene ID (NCBI)	5770
RRID	AB_10642566
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Protocols

Product Specific Protocols
WB protocol for PTP1B antibody 11334-1-AP	Download protocol
IHC protocol for PTP1B antibody 11334-1-AP	Download protocol
IF protocol for PTP1B antibody 11334-1-AP	Download protocol
IP protocol for PTP1B antibody 11334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	J Ginseng Res Ginsenoside compound K reduces ischemia/reperfusion-induced neuronal apoptosis by inhibiting PTP1B-mediated IRS1 tyrosine dephosphorylation Authors - Jing Fu View Article
human	WB	Aging (Albany NY) Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells. Authors - Changying Shi View Article
rat	WB	J Cell Physiol Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p. Authors - Qing-Ping Yao View Article
mouse	WB,IHC	FASEB J PMID: 32100381 Authors - Ping Yang View Article
human	WB	Front Cell Dev Biol Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma. Authors - Juan Du KD Validated View Article
mouse	WB	Eur J Pharmacol Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway Authors - An-Hui Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTP1B Polyclonal antibody

PTP1B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis of Sp2/0 using 11334-1-AP

WB analysis of Jurkat using 11334-1-AP

WB analysis using 11334-1-AP

WB analysis of Jurkat using 11334-1-AP

WB analysis of THP-1 using 11334-1-AP

WB analysis using 11334-1-AP

WB analysis of human testis using 11334-1-AP

WB analysis using 11334-1-AP

WB analysis of HL-60 using 11334-1-AP

WB analysis of PC-3 using 11334-1-AP

WB analysis of human placenta using 11334-1-AP

WB analysis of BxPC-3 using 11334-1-AP

WB analysis of A431 using 11334-1-AP

IP experiment of A431 using 11334-1-AP

IHC staining of human prostate cancer using 11334-1-AP

IF Staining of BxPC-3 using 11334-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ginsenoside compound K reduces ischemia/reperfusion-induced neuronal apoptosis by inhibiting PTP1B-mediated IRS1 tyrosine dephosphorylation

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.

PMID: 32100381

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway