Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 24570-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 24570-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24570-1-AP
WB result of PTPN11 antibody (24570-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells.
WB analysis of MCF-7 using 24570-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 24570-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 24570-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 24570-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 24570-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 24570-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (24570-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24570-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, MCF-7 cells, U-937 cells
Positive IHC detected in	human gliomas tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

24570-1-AP targets SHP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SHP2 fusion protein Ag21472 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight	597 aa, 68 kDa
Observed molecular weight	68 kda
GenBank accession number	BC008692
Gene Symbol	PTPN11
Gene ID (NCBI)	5781
RRID	AB_2879613
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q06124
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 24570-1-AP	Download protocol
IHC protocol for SHP2 antibody 24570-1-AP	Download protocol
IF protocol for SHP2 antibody 24570-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Oncol Lett Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression Authors - Jingru Zhang View Article
human	WB	Heliyon RNA-binding protein THUMPD2 inhibits proliferation and promotes metastasis in epithelial ovarian cancer Authors - Minhui Hua View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 24570-1-AP

WB analysis of U-937 using 24570-1-AP

WB analysis of HeLa using 24570-1-AP

WB analysis of MCF-7 using 24570-1-AP

IHC staining of human gliomas using 24570-1-AP