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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24570-1-AP

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    WB analysis of HEK-293 using 24570-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    WB analysis of U-937 using 24570-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SHP2 Polyclonal antibody (24570-1-AP)

    WB analysis of HeLa using 24570-1-AP

    WB result of PTPN11 antibody (24570-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    WB analysis of MCF-7 using 24570-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SHP2 Polyclonal antibody (24570-1-AP)

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SHP2 Polyclonal antibody (24570-1-AP)

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PTPN11/SHP2 Polyclonal antibody (24570-1-AP)

    IF Staining of MCF-7 using 24570-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (24570-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-937 cells
Positive IHC detected inhuman gliomas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24570-1-AP targets SHP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHP2 fusion protein Ag21472 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kda
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2879613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 24570-1-APDownload protocol
IHC protocol for SHP2 antibody 24570-1-APDownload protocol
IF protocol for SHP2 antibody 24570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Oncol Lett

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression

Authors - Jingru Zhang
View Article
humanWB

Heliyon

RNA-binding protein THUMPD2 inhibits proliferation and promotes metastasis in epithelial ovarian cancer

Authors - Minhui Hua
View Article