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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHP2 Monoclonal antibody, PBS Only

SHP2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3F8A8

Cat no : 66795-1-PBS

Synonyms

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHP2 Monoclonal antibody (66795-1-Ig)

    WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SHP2 Monoclonal antibody (66795-1-Ig)

    WB analysis of HeLa using 66795-1-Ig (same clone as 66795-1-PBS)

    WB result of PTPN11 antibody (66795-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SHP2 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SHP2 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66795-1-PBS targets SHP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13649 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2882139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.