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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHP2 Monoclonal antibody, PBS Only

SHP2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3F8A8

Cat no : 66795-1-PBS

Synonyms

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHP2 Monoclonal antibody (66795-1-Ig)

    WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SHP2 Monoclonal antibody (66795-1-Ig)

    WB analysis of HeLa using 66795-1-Ig (same clone as 66795-1-PBS)

    WB result of PTPN11 antibody (66795-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SHP2 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SHP2 Monoclonal antibody (66795-1-Ig)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using SHP2 Monoclonal antibody (66795-1-Ig)

    FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66795-1-PBS targets SHP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13649 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.