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  • Featured Product
  • KD/KO Validated

SHP2 Polyclonal antibody, PBS Only

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20145-1-PBS

Synonyms

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHP2 Polyclonal antibody (20145-1-AP)

    WB analysis using 20145-1-AP (same clone as 20145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (PTPN11/SHP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SHP2 Polyclonal antibody (20145-1-AP)

    WB analysis using 20145-1-AP (same clone as 20145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SHP2 Polyclonal antibody (20145-1-AP)

    IP experiment of HEK-293 using 20145-1-AP (same clone as 20145-1-PBS)

    IP result of anti-PTPN11/SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:10000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human heart using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human heart using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SHP2 Polyclonal antibody (20145-1-AP)

    IF Staining of HeLa using 20145-1-AP (same clone as 20145-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHP2 antibody (20145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20145-1-PBS targets SHP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13660 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.