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Host
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPN12 Recombinant antibody, PBS Only

PTPN12 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240983C9

Cat no : 83851-6-PBS

Synonyms

PTPG1, PTP PEST, 240983C9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN12 Recombinant antibody (83851-6-RR)

    WB analysis using 83851-6-RR (same clone as 83851-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PTPN12 Recombinant antibody (83851-6-RR)

    IHC staining of human ovary cancer using 83851-6-RR (same clone as 83851-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83851-6-RR

    Affinity and Kinetic Characterization of 83851-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83851-6-PBS is PTPN12 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTPN12 fusion protein Ag35382 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 12
Calculated molecular weight88kDa
Observed molecular weight110-125 kDa
GenBank accession numberNM_002835.3
Gene symbol PTPN12
Gene ID (NCBI) 5782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.